Description
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Product Information
Product Name | Cat.No. | Spec. |
DMEM,no Glucose no L-Glutamine,no HEPES,no sodium pyruvate | G4528-500ML | 500 mL |
Introduction
DMEM (Dulbecco’s Modified Eagle Medium) is a modified medium based on MEM, which has been widely used in the culture of various cells. The DMEM medium was originally designed as low-glucose glucose containing D-Glucose 1000 mg/L, and later developed as high-glucose glucose containing D-Glucose 4500 mg/L. DMEM medium without D-glucose can adjust the glucose concentration at will according to the research needs.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Cells were cultured with 5-10% serum or serum-free additives, depending on the cell type。
This product is sterilized by 0.1μm filter membrane, pH 7.,0-7.4, containing phenol red indicator. No added D-glucose, no L-glutamine and sodium pyruvate, no HEPES buffer system.
Storage and Handling Conditions
Transport at room temperature; Store at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation during use to avoid contamination;;
2. In order to maintain the best use effect, do not freeze and thaw repeatedly.
For Research Use Only!
DMEM, no Glucose Formula
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
Glycine | 30 | 0.4 | Calcium Chloride (CaCl2) (anhyd.) | 200 | 1.80 | |
L-Arginine hydrochloride | 84 | 0.4 | Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) | 0.1 | 0.0002 | |
L-Cystine dihydrochloride | 62.6 | 0.2 | Magnesium Sulfate (MgSO4) (anhyd.) | 97.67 | 0.80 | |
L-Histidine hydrochloride-H2O | 42 | 0.2 | Potassium Chloride (KCl) | 400 | 5.36 | |
L-Isoleucine | 105 | 0.80 | Sodium Bicarbonate (NaHCO3) | 3700 | 44.04 | |
L-Leucine | 105 | 0.80 | Sodium Chloride (NaCl) | 4750 | 81.9 | |
L-Lysine hydrochloride | 146 | 0.8 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) | 108.7 | 0.906 | |
L-Methionine | 30 | 0.2 | Other components | |||
L-Phenylalanine | 66 | 0.4 | D-Glucose (Dextrose) | – | – | |
L-Serine | 42 | 0.4 | Sodium pyruvate | – | – | |
L-Threonine | 95 | 0.8 | HEPES | – | – | |
L-Tryptophan | 16 | 0.08 | Phenol Red | 14.95 | 0.0422 | |
L-Tyrosine disodium salt dihydrate | 103.79 | 0.4 | ||||
L-Valine | 94 | 0.8 | ||||
L-Glutamine | – | – | ||||
L-Alanyl-L-Glutamine | – | – | ||||
Vitamins | ||||||
Choline chloride | 4.0 | 0.0286 | ||||
D-Calcium pantothenate | 4.0 | 0.0084 | ||||
Folic Acid | 4.0 | 0.0091 | ||||
Niacinamide | 4.0 | 0.0328 | ||||
Pyridoxine hydrochloride | 4.0 | 0.0195 | ||||
Riboflavin | 0.4 | 0.0011 | ||||
Thiamine hydrochloride | 4.0 | 0.0119 | ||||
i-Inositol | 7.2 | 0.0389 |
Cat.No.
|
Product Name
|
Spec.
|
Operation
|
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G4528-500ML
|
DMEM, no Glucose, no L-Glutamine, no HEPES, no Sodium Pyruvate
|
500 mL
|
|
G4538-500ML
|
RPMI-1640 Medium, GlutaPlus, no Glucose, no HEPES, no Sodium Pyruvate
|
500 mL
|