13. (2 kinds of no Glucose) DMEM,no Glucose no L-Glutamine,no HEPES,no sodium pyruvate, 500 ml $50

SKU: G4528-500ML
$45.00
In Stock
SKU: G4528-500ML Categories: , Tag:

Description

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Product Information

Product Name Cat.No. Spec.
DMEM,no Glucose no L-Glutamine,no HEPES,no sodium pyruvate G4528-500ML 500 mL

 


Introduction

DMEM (Dulbecco’s Modified Eagle Medium) is a modified medium based on MEM, which has been widely used in the culture of various cells. The DMEM medium was originally designed as low-glucose glucose containing D-Glucose 1000 mg/L, and later developed as high-glucose glucose containing D-Glucose 4500 mg/L. DMEM medium without D-glucose can adjust the glucose concentration at will according to the research needs.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Cells were cultured with 5-10% serum or serum-free additives, depending on the cell type。

This product is sterilized by 0.1μm filter membrane, pH 7.,0-7.4, containing phenol red indicator. No added D-glucose, no L-glutamine and sodium pyruvate, no HEPES buffer system.

 


Storage and Handling Conditions

Transport at room temperature; Store at 2-8℃, valid for 12 months.


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation during use to avoid contamination;;

2. In order to maintain the best use effect, do not freeze and thaw repeatedly.

 

For Research Use Only!

DMEM, no Glucose Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 30 0.4 Calcium Chloride (CaCl2) (anhyd.) 200 1.80
L-Arginine hydrochloride 84 0.4 Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) 0.1 0.0002
L-Cystine dihydrochloride 62.6 0.2 Magnesium Sulfate (MgSO4) (anhyd.) 97.67 0.80
L-Histidine hydrochloride-H2O 42 0.2 Potassium Chloride (KCl) 400 5.36
L-Isoleucine 105 0.80 Sodium Bicarbonate (NaHCO3) 3700 44.04
L-Leucine 105 0.80 Sodium Chloride (NaCl) 4750 81.9
L-Lysine hydrochloride 146 0.8 Sodium Phosphate monobasic (NaH2PO4) (anhyd.) 108.7 0.906
L-Methionine 30 0.2 Other components
L-Phenylalanine 66 0.4 D-Glucose (Dextrose)
L-Serine 42 0.4 Sodium pyruvate
L-Threonine 95 0.8 HEPES
L-Tryptophan 16 0.08 Phenol Red 14.95 0.0422
L-Tyrosine disodium salt dihydrate 103.79 0.4
L-Valine 94 0.8
L-Glutamine
L-Alanyl-L-Glutamine
Vitamins
Choline chloride 4.0 0.0286
D-Calcium pantothenate 4.0 0.0084
Folic Acid 4.0 0.0091
Niacinamide 4.0 0.0328
Pyridoxine hydrochloride 4.0 0.0195
Riboflavin 0.4 0.0011
Thiamine hydrochloride 4.0 0.0119
i-Inositol 7.2 0.0389

 

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