12. (4 kinds of DMEM/F-12,) DMEM/F-12 GlutaPlus,no HEPES, 500 ml $40

SKU: G4610-500ML-1
$45.00
In Stock

Description

Please click here to see the video. thanks

and another video to see the product quality. 

Product Information

Product Name Cat. No. Spec.
DMEM/F-12, GlutaPlus, no HEPES G4610-500ML 500 mL

 


Product Description/Introduction

DMEM/F-12 medium was modified by mixing DMEM with Ham’s F-12 medium at a ratio of 1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more micronutrients, which is a widely used basal medium for supporting the growth of many different mammalian cells, as well as for the culture of mammalian cells at low serum levels and for clonal density culture. DMEM/F-12 has also been used as a base medium for the development of serum-free medium.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 17.5 mM D-glucose, 2.5 mM L-Alanyl-L-Glutamine, 0.5 mM sodium pyruvate, phenol red indicator, no HEPES.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4610 DMEM/F-12 Formula

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Inorganic salts
Glycine 18.75 0.250 Calcium Chloride (CaCl2)(anhyd.) 116.6 1.050
L-Alanine 4.45 0.050 Cupric Sulfate (CuSO4·5H2O) 0.0013 0.0000052
L-Arginine monohydrochloride 147.5 0.699 Ferric Nitrate (Fe(NO3)3·9H2O) 0.05 0.0001238
L-Asparagine monohydrate 7.5 0.050 Ferrous Sulfate (FeSO4·7H2O) 0.417 0.0015
L-Asparagine 6.65 0.050 Magnesium chloride (MgCl2)(anhyd.) 28.64 0.301
L-Cysteine hydrochloride hydrate 17.56 0.100 Magnesium sulfate (MgSO4) (anhyd.) 48.84 0.407
L-Cystine dihydrochloride 31.29 0.100 Potassium Chloride (KCl) 311.8 4.157
L-Glutamic acid 7.35 0.050 Sodium bicarbonate(NaHCO3) 2438 29.02
L-Alanyl-L-Glutamine 543.0 2.500 Sodium chloride (NaCl) 6995.5 120.6
L-Histidine hydrochloride monohydrate 31.48 0.150 Disodium (hydrogen) phosphate(Na2HPO4)(anhyd.) 71.02 0.500
L-Isoleucine 54.47 0.416 Sodium dihydrogen phosphate(NaH2PO4)(anhyd.) 54.34 0.453
L-Leucine 59.05 0.451 Zinc Sulfate(ZnSO4·7H2O) 0.432 0.0015
L-Lysine hydrochloride 91.25 0.499 Other components
L-Methionine 17.24 0.116 D-Glucose (Dextrose) 3151 17.51
L-Phenylalanine 35.48 0.215 Hypoxanthine Na 2.39 0.015
L-Proline 17.25 0.150 Linoleic Acid 0.042 0.00015
L-Serine 26.25 0.250 Lipoic acid 0.105 0.00051
L-Threonine 53.45 0.449 Phenol Red 8.1 0.022
L-Tryptophan 9.02 0.044 Putrescine dihydrochloride 0.081 0.00050
L-Tyrosine disodium salt 55.79 0.214 Thymidine 0.365 0.00151
L-Valine 52.85 0.452 Sodium pyruvate 55 0.500
Vitamins HEPES
Biotin 0.0035 0.0000143
Choline chloride 8.98 0.06414
D-Calcium pantothenate 2.24 0.00470
Folic Acid 2.65 0.00601
Niacinamide 2.02 0.01656
Pyridoxine hydrochloride 2.013 0.00977
Riboflavin 0.219 0.00058
Thiamine hydrochloride 2.17 0.00644
Vitamin B12 0.68 0.00050
i-Inositol 12.6 0.07
Product recommendation
Cat.No.
Product Name
Spec.
Operation
G4610-500ML
DMEM/F-12, GlutaPlus, no HEPES
500 mL
G4611-500ML
DMEM/F-12, no L-Glutamine, no HEPES
500 mL
G4612-500ML
DMEM/F-12, GlutaPlus, HEPES
500 mL
G4613-500ML
DMEM/F-12, HEPES, no L-Glutamine
500 mL

Shopping Cart
No products in the cart.