119-2. ATP Luminescence Cell Viability Assay Kit, $ 600

ATP Luminescence Cell Viability Assay Kit

Product Information:

• Product Name: ATP Luminescence Cell Viability Assay Kit
• Product Number:
  • G1612-100T (10 mL)
  • G1612-1000T (100 mL)
• Specification: See above
• Price: See above

Product Description:

The ATP Luminescence Cell Viability Assay Kit is designed to assess cell viability based on the quantification of ATP (adenosine triphosphate) levels in cells. ATP is a vital molecule in various cellular processes and is often referred to as the “energy currency” of cells. The level of ATP correlates with the cellular state and number, making it a valuable indicator of cell viability.

This assay kit utilizes the principle of ATP-dependent luciferase-catalyzed production of luminescence. It provides a highly sensitive and accurate method to detect and evaluate cellular ATP levels. The luminescence-based approach offers advantages over traditional absorbance-based methods, such as CCK-8 and MTT, including higher sensitivity and a broader detection range. The assay is easy to use and requires no washing, media changes, or extended incubation times, enabling rapid assessment of cell number and viability.

Storage and Transportation:

• Shipped with dry ice
• Store at -80°C in a dark environment for a shelf life of 12 months
• Alternatively, store at -20°C in a dark environment with recommended usage within 6 months

Kit Components:

Operating Instructions:

1. Prepare cell samples by seeding them in a 96-well plate at a desired density. Use a white, non-transparent plate. If using a transparent plate, create spacing between samples to minimize interference.
2. Prepare ATP standard samples (optional) by diluting ATP standards (not provided) in PBS or cell culture media and adding 100 μL to each well.
3. Cell Viability Assay:

   3.1. Thaw and equilibrate the ATP Luminescence Cell Viability Assay Reagent to room temperature.

   3.2. Remove the cell culture plate and allow it to equilibrate at room temperature for 5-10 minutes.

   3.3. Add 100 μL of the ATP Luminescence Cell Viability Assay Reagent directly to each well.

   3.4. Gently shake or tap the plate to ensure thorough mixing.

   3.5. After a 3-minute incubation, measure luminescence using a luminometer, a multi-mode plate reader with a luminescence module, or other appropriate instruments.

4. Data Analysis:

   4.1. Relative Cell Number (Increase) Analysis: Determine the cell number based on the intensity of the luminescent signal.

   4.2. Cell Viability Calculation (Cell Proliferation or Cell Toxicity):

   

   Note: A (Treated Group): Treated cells + ATP Luminescence Cell Viability Assay Reagent A1 (Untreated Group): Untreated cells + ATP Luminescence Cell Viability Assay Reagent A0 (Blank Control Group): Cell culture media (no cells) + ATP Luminescence Cell Viability Assay Reagent

   4.3. ATP Content Quantitative Analysis (optional):

   

   • Calculate the slope (a) and intercept (b) of the ATP standard curve.
   • Use the formula to calculate the X value (ATP content) for the assay system.

   

   • Calculate the ATP content in the sample.

Note:

• Follow the provided product manual for detailed experimental protocols and guidelines.
• Maintain appropriate laboratory safety practices while handling reagents and working with live cells.
• The assay is suitable for a wide range of applications, including drug screening, toxicity assessment, and proliferation studies.
• This product is intended for research use only and is not for clinical diagnosis or therapeutic purposes.