11-2. McCoy’s 5A (Modified) Medium, GlutaPlus, HEPES, 500 ml $40

SKU: G4541-500ML
$45.00
In Stock
SKU: G4541-500ML Categories: , Tag:

Description

Please click here to see the video. thanks

and another video to see the product quality. 

Product Information

Product Name Cat. No. Spec.
McCoy’s 5A (Modified) Medium, GlutaPlus, HEPES G4541-500ML 500 mL

 


Product Description

McCoy’s 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. McCoy’s 5A modified contains a high concentration of D-glucose, bacterial peptone and reduced glutathione compared to other media.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 16.67 mM D-glucose, 1.5 mM L-Alanyl-L-Glutamine, 25 mM HEPES buffer system, phenol red indicator, no sodium pyruvate.

HEPES is an excellent biological buffering agent with no toxic effect on cells, and the medium supplemented with HEPES can maintain a constant pH range for a long time.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

 

G4541 McCoy’s 5A, modified, HEPES

 

Components Concentration Components Concentration
mg/L mM mg/L mM
Amino acids Thiamine hydrochloride 0.20 0.000593472
Glycine 7.5 0.1000 Vitamin B12 2.00 0.001476015
L-Alanine 13.9 0.156 i-Inositol 36.0 0.2000
L-Arginine hydrochloride 42.1 0.1995 Inorganic salts
L-Asparagine monohydrate 51.14 0.3409 Calcium Chloride (CaCl2)(anhyd.) 100 0.901
L-Asparagine 19.97 0.1502 Magnesium sulfate(MgSO4)(anhyd.) 200 0.813
L-Cysteine hydrochloride hydrate 31.5 0.2603 Potassium Chloride (KCl) 400 5.333
L-Glutamic acid 22.1 0.1503 Sodium bicarbonate(NaHCO3) 2200 26.190
L-Alanyl-L-Glutamine 326.1 1.5014 Sodium chloride (NaCl) 5100 87.931
L-Histidine hydrochloride monohydrate 20.96 0.0998 Sodium Phosphate monobasic(NaH2PO4)(anhyd.) 504 4.203
L-Isoleucine 19.7 0.1504 Other components
L-Leucine 39.36 0.3005 Peptone 600 Infinity
L-Lysinehydrochloride 39.36 0.3005 D-Glucose (Dextrose) 3000 16.67
L-Lysine Hydrochloride 36.5 0.1995 Reduced glutathione 0.5 0.0016
L-Methionine 14.9 0.1000 Phenol Red 9.4 0.0266
L-Phenylalanine 16.5 0.1000 HEPES 5958 25
L-Proline 17.3 0.1504
L-Serine 26.3 0.2505
L-Threonine 17.9 0.1504
L-Tryptophan 3.06 0.0152
L-Tyrosine disodium salt 26.1 0.1000
L-Valine 17.6 0.1504
Vitamins
L-Ascorbic acid 0.50 0.002840909
Biotin 0.20 0.000819672
Choline chloride 5.00 0.035714287
D-Calcium pantothenate 0.20 0.000419287
Folic Acid 10.00 0.022675738
Niacinamide 0.50 0.00409836
Nicotinic acid 0.50 0.004065041
Para-Aminobenzoic Acid 1.00 0.00729927
Pyridoxal hydrochloride 0.50 0.002450981
Pyridoxine hydrochloride 0.50 0.002427185
Riboflavin 0.20 0.000531915

 

Product recommendation
Cat.No.
Product Name
Spec.
Operation
G4540-500ML
McCoy’s 5A (Modified) Medium, GlutaPlus, no HEPES
500 mL
G4541-500ML
McCoy’s 5A (Modified) Medium, GlutaPlus, HEPES
500 mL

Shopping Cart
No products in the cart.