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10. 20× Citric Acid Antigen-Retrieval Solution (pH 6.0); 250ml $145
$145.00
In Stock
Description
Product Information
| Product Name | Cat.No. | Spec. |
| 20× Citric Acid Antigen-Retrieval Solution (pH 6.0) | G1202-250ML | 250 mL |
Description
After cells or tissues are fixed with paraformaldehyde, formalin or other aldehyde reagents, the formation of aldehyde bond and carboxymethyl bond of antigen determinant, as well as the cross-linking of protein quality control, cause the change of protein spatial structure, so that the antigen determinant is closed, and the binding point between antigen and antibody is reduced. Finally, the positive detection rate and staining intensity of antigen during immune labeling are relatively weak, showing false negative or low positive rate. The protein cross-linking caused by aldehydes is reversible. Under certain conditions, such as high temperature or protease hydrolysis, the original conformation of the protein can be restored. This process is antigen retrieval. Antigen repair is a key step in immunohistochemistry. There are many methods for antigen repair, and different methods can be selected according to the characteristics of the tissue type. Some are suitable for thermal repair, while others are suitable for enzymatic repair. The pH value of antigen repair solution also has many different choices due to the characteristics of antigen. The company provides a variety of antigen repair solutions with different pH values, which can meet the needs of a variety of antigen repair.
This product 20 × Citric acid antigen repair solution (pH 6.0) is a concentrated citric acid buffer solution. The main component of the product is 200 mM citric acid buffer solution. After 20 times of dilution, a citric acid buffer solution with pH 6.0 and concentration of 10 mM can be obtained, which can be used for antigen repair.
Storage and Handling Conditions
Normal temperature storage and transportation; Valid for 12 months.
Assay Protocol
Every 10 mL of 20× citric acid antigen repair solution (pH 6.0) was mixed with 190 mL distilled water to obtain 1× citric acid antigen repair solution with a concentration of 10 mM and a pH of 6.0.
Note:
1. This product is a concentrated solution, which needs to be diluted before use. The diluted buffer is recommended to be used up on the same day and can be stored briefly at 4 ° C.
2. Select appropriate antigen repair solution according to experimental requirements and antigen characteristics. If you need alkaline repair solution, refer to G1203, G1206.
3. Wear a lab coat and disposable gloves during operation.
For Research Use Only!
