1. Serum/Protein-Free Non-gradient Cell Freezing Medium (with DMSO)100ml, $350

Product Information

Product Description

Serum/Protein-Free Non-gradient Cell Freezing Medium is a chemically defined, serum-free, protein-free, DMSO-containing, ready-to-use non-gradient cryopreservation medium. This product is chemically defined and serum-free, which can effectively avoid the unstable results of serum batch instability and unknown composition on cell freezing. Serum-free and protein-free, the potential effects of certain proteins on some specific cells can be avoided and applied to serum-free culture cells or expressed cells for cryopreservation. In addition, compared with traditional cryopreservation medium, this product take the non-gradient freezing, only need to resuspend the cells with this product and put them directly into -80℃ refrigerator, eliminating the operation of tedious gradient freezing.

This product has been continuously optimized and debugged by our technical team, and while it has the above advantages, it greatly weakens the crystallization process of water, protects cells from solute damage, and effectively improves viability and cell recovery after thawing. Suitable for multi-type, multi-source, and multi-purpose cell freezing.

Storage and Shipping Conditions

Ship with wet ice; Store away from light at 2-8℃, valid for 12 months.

Product Contents

Assay Protocol

1. Cells in logarithmic growth phase are collected in centrifuge tube according to the conventional method.

2. Centrifuge cells for 3-5 minutes at 800-1200 rpm/min. Carefully aspirate the supernatant.

3. Add Serum/Protein-Free Non-gradient Cell Freezing Medium, gently resuspend cells, cell density is controlled at 8×105- 5×106/mL.

4. Transfer the cell-containing cryopreservation medium in the appropriate volume (1 mL/piece  recommended) to the cell cryotube.

5. Freeze the cells directly in -80℃ refrigerator.

6. Move into liquid nitrogen for long-term storage after 24 h, also can be stored in -80℃ refrigerator for short-term storage.

Note

1. Gently shake the reagent before use to ensure even distribution of each component.

2. Please use up the product within 3 months after opening, also can be divided and put in -20℃ for long-term storage.

3. Make sure the cells are in good condition before freezing, and make sure the corresponding markers are made on the cell cryotube. Use marker pen that are resistant to organic solvents to avoid loss of markers on the cell cryotube.

4. For your safety and health, please wear safety glasses, gloves or protective clothing.

For Research Use Only!