1. Ice-Bath Free Fast Transfer Buffer, 1 L (10* Concentrated); $65

Product Information

Product Description

This product is a safe and harmless buffer for rapid wet transfer in Western Blot, with efficient and rapid transfer of proteins to blotting membranes in 15-30 min without the use of methanol.

This product is a 10-fold concentrated solution and should be diluted 10 times before use. Mix 100 mL of this product with 200 mL of anhydrous ethanol or methanol, then add deionised water or distilled water to fix the volume to 1 L. Mix well to obtain 1 x working solution.

Rapid transfer without ice bath: With rapid transfer buffer, the transfer of proteins from gels to membranes such as PVDF or nitrocellulose (NC) membranes can be carried out at a constant flow membrane of 400 mA for 15-30 min, with lower heat production than with conventional transfer buffer.

Storage and Shipping Conditions

Ship at room temperature; Store in a dry place, valid for 24 months.

Assay Protocol

Complete the sandwich structure required for transfer, from the negative electrode (black side) to the positive electrode (red side) in the order of sponge, filter paper, gel, blotting film, filter paper, sponge, and then transfer to the tank, set a constant current of 400 mA, transfer time of 15-30 min (this condition is for 1.0mm thick gel, if the gel thickness is 0.75mm , appropriately reduce the transfer time; If the gel thickness is 1.5 mm, extend the transfer time appropriately) to complete the protein transfer.

Note

1. This product is suitable for the transfer of PAGE gel of Tris-Gly, Bis-Tris and other buffer systems.

2. PVDF membrane should be infiltrated with absolute ethanol for about 30 s before use.

3. The transfer current is recommended to be set at a constant current of 400 mA. Generally, 5-15 min for proteins below 20 kDa, 15-25 min for 20-150 kDa and 25-35 min for proteins above 150 kDa.

4. It does not require ice bath for transfer within 30 min. If the transfer is over 30 min, the ice bath is required.

5. Due to the different brand and model of the power supply used for the transfer, the maximum current and the power varies greatly, some power supplies set at a constant current of 400 mA will exceed the maximum power and overload protection will occur, so it is necessary to adjust the current downwards or use a power supply with more power.

6. The temperature of the transfer buffer, gel parameters (such as thickness, acrylamide concentration, ion concentration), filter paper thickness, volume of transfer buffer and times of use of transfer buffer may affect the current and voltage; In case of overload, please reduce the current and prolong the transfer time.

7. The normal color of this product is colorless and transparent. If light brown or yellowish brown appears, it should be discarded promptly.

8. For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!