8. Fluo-4 AM Calcium Ion Fluorescence Assay Kit, $400

Description

Fluo-4 AM Calcium Ion Fluorescence Assay Kit

Product Information:

• Product Name: Fluo-4 AM Calcium Ion Fluorescence Assay Kit
• Product Number: G1724-100T
• Specification: 100 tests

Product Description:

The Fluo-4 AM Calcium Ion Fluorescence Assay Kit is designed for the detection of intracellular calcium ion (Ca²⁺) levels in live cells using the fluorescent probe Fluo-4 AM. Fluo-4 AM is a cell-permeable dye that exhibits increased fluorescence upon binding to calcium ions, allowing for real-time monitoring of changes in intracellular calcium concentrations. Changes in calcium ion concentrations are associated with various cellular processes, including cell signaling, contraction, secretion, and more.

This assay kit includes Fluo-4 AM as the main probe, along with specialized reagents and instructions for the sensitive and reliable detection of intracellular calcium ion levels in biological samples. The emitted fluorescence signal is proportional to the amount of bound calcium ions, enabling the study of calcium-related cellular events.

Storage and Transportation:

• Shipped on dry ice
• Store at -20°C in a dark environment
• Shelf life: 12 months

Kit Components:

Note: The number of tests specified corresponds to the 96-well plate detection system.

Operating Instructions:

1. Prepare the cells or tissue samples as needed for your experiment.
2. Prepare the Fluo-4 AM Working Solution: Follow the instructions provided in the product manual to prepare the Fluo-4 AM Working Solution.
3. Staining and Labeling:3.1. Remove the original cell culture medium and wash the cells or tissue samples with PBS or appropriate buffer.3.2. Remove the wash buffer and add the prepared Fluo-4 AM Working Solution. Incubate in the dark at room temperature or 37°C for a specified time.

   3.3. After the incubation period, wash the samples with PBS or buffer to remove excess probe.

4. Fluorescence Detection: Use appropriate instruments such as fluorescence microscopes, microplate readers, flow cytometers, etc., to detect changes in intracellular calcium ion levels through the increased fluorescence signal of Fluo-4 AM.

Note:

1. The recommended staining conditions and incubation times may vary based on the sample type and experimental design. Adjustments can be made accordingly.
2. Minimize exposure to light during the staining and detection process to avoid potential interference with fluorescence.
3. Wear appropriate laboratory attire and gloves for your safety and health.
4. This product is intended for research use only and is not for clinical diagnosis or treatment.