28. MEM, NEAA, no L-Glutamine, no HEPES

SKU: G4550-500ML
$40.00
In Stock
SKU: G4550-500ML Categories: ,

Description

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Product Information

Product Name Cat. No. Spec.
MEM, NEAA, no L-Glutamine, no HEPES G4550-500ML 500 mL

 


Product Description/Introduction 

MEM (Minimum Essential Medium), also known as minimum basic medium, low limit Eagle medium, is one of the most commonly used basic mediums in animal cell culture. MEM contains only 12 amino acids, 8 vitamins and basic inorganic salts, with a simple nutrient composition, is mainly used for the culture of walled cells, but can also be used for other types of cell culture after the formulation is revised.

MEM which contains NEAA (non-essential amino acids) is based on MEM with the addition of 7 NEAA, including L-Alanine, L-Glutamic acid, L-Asparagine, L-Aspartic acid, L-Proline, L-Serine and Glycine, to reduce the side effects of cells’ own synthesis of non-essential amino acids during cell culture and effectively promote cell proliferation and metabolism.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 5.5 mM D-glucose, phenol red indicator, without L-glutamine, sodium pyruvate and HEPES.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves or protective clothing.

 

For Research Use Only!

G4550 MEM, with NEAA

Components Concentration  Components Concentration 
mg/L mM mg/L mM
Amino acids Inorganic salts
L-Arginine hydrochloride 126.0 0.5972 Calcium Chloride (CaCl2)(anhyd.) 200 1.802
L-Cystine 2HCl 31.0 0.09904 Magnesium Sulfate (MgSO4) (anhyd.) 200 0.813
L-Glutamine Potassium Chloride (KCl) 400 5.333
L-Histidine hydrochloride-H2O 42.0 0.2 Sodium Bicarbonate (NaHCO3) 2200 26.19
L-Isoleucine 52.0 0.3969 Sodium Chloride (NaCl) 6800 117.2
L-Leucine 52.0 0.3969 Sodium Phosphate monobasic (NaH2PO4) (anhyd.) 122 1.014
L-Lysine hydrochloride 73.0 0.3989 Other components
L-Methionine 15.0 0.1007 D-Glucose (Dextrose) 1000 5.556
L-Phenylalanine 32.0 0.1939 Phenol Red 9.4 0.02657
L-Threonine 48.0 0.4034 HEPES
L-Tryptophan 10.0 0.04902
L-Tyrosine disodium salt dihydrate 52.0 0.1992
L-Valine 46.0 0.3932
Glycine 8.0 0.1067
L-Alanine 9.0 0.1011
L-Asparagine monohydrate 14.8 0.09848
L-Asparagic acid 13.0 0.09774
L-Aminoglutaric acid 15.0 0.1020
L-Proline 12.0 0.1043
L-Serine 11.0 0.1048
Vitamins
Choline chloride 1.0 0.007143
D-Calcium pantothenate 1.0 0.002096
Folic Acid 1.0 0.002268
Niacinamide 1.0 0.008197
Pyridoxine hydrochloride 1.0 0.004902
Riboflavin 0.1 0.0002660
Thiamine hydrochloride 1.0 0.002967
i-Inositol 2.0 0.01111

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