11-2. McCoy’s 5A (Modified) Medium, GlutaPlus, HEPES, 500 ml $40
Description
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Product Information
Product Name | Cat. No. | Spec. |
McCoy’s 5A (Modified) Medium, GlutaPlus, HEPES | G4541-500ML | 500 mL |
Product Description
McCoy’s 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. McCoy’s 5A modified contains a high concentration of D-glucose, bacterial peptone and reduced glutathione compared to other media.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.
This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 16.67 mM D-glucose, 1.5 mM L-Alanyl-L-Glutamine, 25 mM HEPES buffer system, phenol red indicator, no sodium pyruvate.
HEPES is an excellent biological buffering agent with no toxic effect on cells, and the medium supplemented with HEPES can maintain a constant pH range for a long time.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves and protective clothing.
For Research Use Only!
G4541 McCoy’s 5A, modified, HEPES
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Thiamine hydrochloride | 0.20 | 0.000593472 | |||
Glycine | 7.5 | 0.1000 | Vitamin B12 | 2.00 | 0.001476015 | |
L-Alanine | 13.9 | 0.156 | i-Inositol | 36.0 | 0.2000 | |
L-Arginine hydrochloride | 42.1 | 0.1995 | Inorganic salts | |||
L-Asparagine monohydrate | 51.14 | 0.3409 | Calcium Chloride (CaCl2)(anhyd.) | 100 | 0.901 | |
L-Asparagine | 19.97 | 0.1502 | Magnesium sulfate(MgSO4)(anhyd.) | 200 | 0.813 | |
L-Cysteine hydrochloride hydrate | 31.5 | 0.2603 | Potassium Chloride (KCl) | 400 | 5.333 | |
L-Glutamic acid | 22.1 | 0.1503 | Sodium bicarbonate(NaHCO3) | 2200 | 26.190 | |
L-Alanyl-L-Glutamine | 326.1 | 1.5014 | Sodium chloride (NaCl) | 5100 | 87.931 | |
L-Histidine hydrochloride monohydrate | 20.96 | 0.0998 | Sodium Phosphate monobasic(NaH2PO4)(anhyd.) | 504 | 4.203 | |
L-Isoleucine | 19.7 | 0.1504 | Other components | |||
L-Leucine | 39.36 | 0.3005 | Peptone | 600 | Infinity | |
L-Lysinehydrochloride | 39.36 | 0.3005 | D-Glucose (Dextrose) | 3000 | 16.67 | |
L-Lysine Hydrochloride | 36.5 | 0.1995 | Reduced glutathione | 0.5 | 0.0016 | |
L-Methionine | 14.9 | 0.1000 | Phenol Red | 9.4 | 0.0266 | |
L-Phenylalanine | 16.5 | 0.1000 | HEPES | 5958 | 25 | |
L-Proline | 17.3 | 0.1504 | ||||
L-Serine | 26.3 | 0.2505 | ||||
L-Threonine | 17.9 | 0.1504 | ||||
L-Tryptophan | 3.06 | 0.0152 | ||||
L-Tyrosine disodium salt | 26.1 | 0.1000 | ||||
L-Valine | 17.6 | 0.1504 | ||||
Vitamins | ||||||
L-Ascorbic acid | 0.50 | 0.002840909 | ||||
Biotin | 0.20 | 0.000819672 | ||||
Choline chloride | 5.00 | 0.035714287 | ||||
D-Calcium pantothenate | 0.20 | 0.000419287 | ||||
Folic Acid | 10.00 | 0.022675738 | ||||
Niacinamide | 0.50 | 0.00409836 | ||||
Nicotinic acid | 0.50 | 0.004065041 | ||||
Para-Aminobenzoic Acid | 1.00 | 0.00729927 | ||||
Pyridoxal hydrochloride | 0.50 | 0.002450981 | ||||
Pyridoxine hydrochloride | 0.50 | 0.002427185 | ||||
Riboflavin | 0.20 | 0.000531915 |
Cat.No.
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Product Name
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Spec.
|
Operation
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G4540-500ML
|
McCoy’s 5A (Modified) Medium, GlutaPlus, no HEPES
|
500 mL
|
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G4541-500ML
|
McCoy’s 5A (Modified) Medium, GlutaPlus, HEPES
|
500 mL
|