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For long-term in vivo safety studies, rabbits with implanted HHF for different durations were euthanized. After fixation of joint tissues containing HHF, sections were subjected to morphological staining using HE staining solution (Servicebio, G1005) and Masson’s trichrome staining solution (Servicebio, G1006). For immunohistochemical staining, the primary antibody used was Anti-F4/80 Rabbit pAb (Servicebio, GB11027), the secondary antibody was Fluorescein-labeled Goat Anti-Rabbit IgG (H+L) (Servicebio, GB25303), and cell nuclei were labeled using DAPI staining reagent (ready-to-use) (Servicebio, G1012)
| Catalog Number | Name | Specification | Price (¥) |
| G1005 | HE Staining Solution | 500 mL | Lower and lower |
| G1004 | Hematoxylin Staining Solution | 500 mL | |
| G1039 | Hematoxylin Differentiation Solution | 500 mL | |
| G1040 | Hematoxylin Bluing Reagent | 500 mL | |
| G1050 | TRAP Staining Reagent Kit | 50 T | |
| G1006 | Masson’s Trichrome Staining Solution | 100 mL | |
| GB11027 | Anti-F4/80 Rabbit pAb | 100 μL | |
| GB25303 | Alexa Fluor 488-labeled Goat Anti-Rabbit IgG | 100 μL | |
| G1012 | DAPI Staining Reagent (Ready-to-use) | 100 mL | |
| G1407 | Anti-fade Mounting Medium with DAPI | 25 mL |